ANTIOKSIDATIVNA AKTIVNOST I SADRŽAJ ODABRANIH MIKRONUTRIJENATA U LISTOVIMA VRSTE Asplenium ceterach
DOI:
https://doi.org/10.63356/asb.2026.003Ključne reči:
sposobnost uklanjanja DPPH, redukciona sposobnost, sposobnost uklanjanja hidroksilnog radikala, sposobnost uklanjanja H2O2Apstrakt
Cilj ovog istraživanja je bio ispitivanje sadržaja fenolnih jedinjenja i antioksidativnog kapaciteta etanolnog ekstrakta listova vrste Asplenium ceterach, sakupljene u Republici Srpskoj (BiH). Takođe, cilj rada je bio i određivanje koncentracije odabranih mikronutrijenata (Fe, Cu, Zn, Mn) u listovima ove vrste. Dobijeni rezultati su pokazali da je koncentracija ukupnih fenolnih jedinjenja (TPC) u ekstraktu iznosila 16,08 ± 0,08 mg GAE/gDW. Ekstrakt je pokazao i sposobnost uklanjanja DPPH (2,2-diphenyl-1-picrylhydrazyl) radikala sa IC₅₀ vrijednošću od 29,28 ± 2,20 mg/mL. Kapacitet uklanjanja vodonik peroksida bio je značajno niži (IC₅₀ = 1420,56 ± 35,72 μg/mL), što ukazuje na umjerenu efikasnost za uklanjanje ove reaktivne vrste kiseonika. Ekstrakt je takođe pokazao sposobnost uklanjanja hidroksilnih radikala, pri čemu je u ispitivanim uslovima izmjereno 56,26 ± 1,05% preostalog •OH radikala nakon primjene ekstrakta. Pri koncentraciji od 500 µg/mL TPC, ekstrakt je redukovao Fe³⁺ sa vrijednošću od 1420,20 ± 53,67 mmol/L Fe²⁺ ekvivalenta, dok je njegov kapacitet redukcije Cu²⁺ iznosio 423,14 ± 29,20 µg Trolox/mL pri TPC koncentraciji od 300 µg/mL. Koncentracije mikronutrijenata u listovima vrste A. ceterach bile su sljedeće: Fe (164,57 ± 4,08 mg/kg) > Zn (15,61 ± 0,43 mg/kg) > Mn (12,39 ± 0,19 mg/kg) > Cu (1,62 ± 0,12 mg/kg). Naši in vitro rezultati ukazuju na to da bi etanolni ekstrakt listova vrste A. ceterach mogao poslužiti kao potencijalni prirodni izvor antioksidativnih jedinjenja.
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