ANTIOKSIDATIVNI ODGOVOR BILJAKA KUKURUZA NA STRES IZAZVAN SOLJU
DOI:
https://doi.org/10.7251/ASB230402013PKljučne reči:
salinitet, ZP 555, proteini, GSH, POX, APX, CATApstrakt
Cilj rada je bio da se ispita uticaj povećane koncentracije NaCl na koncentraciju proteina i antioksidativni sistem korijena i listova kukuruza. Biljke kukuruza (hibrid ZP 555) su gajene hidroponično i tretirane sa NaCl u koncentracijama od 50 i 150 mM tokom šest dana.
Od antioksidativnih parametara određeni su: koncentracija glutationa (GSH) i aktivnost antioksidativnih enzima: peroksidaze klase III (POX, EC 1.11.1.7), askorbat peroksidaza (APX, EC 1.11.1.11) i katalaza (CAT, EC 1.11.1.6). Nakon tretmana sa 50 mM NaCl, koncentracija proteina u listu i korijenu se smanjila, dok je nakon tretmana sa 150 mM NaCl izmjereno povećanje koncentracija proteina, ali samo u listu. Povećanje koncentracije GSH detektovano je za obje koncentracije NaCl u listu, a u korijenu samo za nižu koncentraciju, u poređenju sa kontrolom. Aktivnost POX je značajno porasla samo u listovima tretiranim sa 150 mM NaCl, dok je pri nižoj koncentraciji aktivnost POX smanjena, kako u listovima kukuruza tako i u korijenu. Pet izoformi rPOX detektovano je nativnom gel elektroforezom u kontroli, dok izoforma rPOX5 nije detektovana u tretiranim uzorcima. Dvije lPOX izoforme su detektovane u kontrolnim i tretiranim uzorcima lista. Nativna elektroforeza je pokazala prisustvo jedne CAT izoforme samo u listovima, i u kontrolnim i u tretiranim uzorcima. Najveća CAT aktivnost je izmjerena pri nižoj koncentraciji NaCl. Na osnovu dobijenih rezultata može se zaključiti da salinitet mijenja antioksidativni sistem u listovima i korijenu kukuruza. Pored toga, na osnovu mjerenih parametara može se zaključiti da hibrid ZP 555 ima umjerenu tolerantnost na ispitivane nivoe saliniteta.
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