ANTIOXIDATIVE RESPONSE OF MAIZE TO SALT-INDUCED STRESS
DOI:
https://doi.org/10.7251/ASB230402013PKeywords:
salinity, ZP 555, proteins, GSH, POX, APX, CATAbstract
The aim of the work was to examine the influence of high concentrations of NaCl on protein concentration and the antioxidant system of maize roots and leaves. Maize plants (hybrid ZP 555) were treated with sodium chloride (NaCl) in concentrations of 50 and 150 mM for six days, hydroponically. Among the antioxidant parameters, the concentration of glutathione (GSH) and the activity of antioxidant enzymes: Class III peroxidase (POX, EC 1.11.1.7), ascorbate peroxidase (APX EC 1.11.1.11), and catalase (CAT, EC 1.11.1.6) were determined. When treated with 50 mM NaCl, the concentration of proteins in the leaves and roots decreased while at 150 mM NaCl the concentration of proteins increased but only in the leaves. An increase in GSH concentration was detected at both concentrations of NaCl in the leaf, and in the root only at 50 mM NaCl, compared to the control. The activity of POX increased significantly only in the leaves treated with the higher concentration of NaCl, while at lower concentrations it decreased, in both leaves and roots. Five rPOX isoforms were detected by native gel electrophoresis in the control, while no rPOX5 isoform was detected in the treated samples. Two lPOX isoforms were detected in both control and treated samples. Native electrophoresis showed the presence of one CAT isoform only in leaves, in both control and treated samples. The highest CAT activity was measured at the lower NaCl concentration. Based on the obtained results, it can be concluded that salinity changes the antioxidant system in the leaves and roots of maize. Also, based on the measured parameters, it can be concluded that the ZP 555 hybrid has a moderate tolerance to the tested salinity levels.
References
AbdElgawad, H., Zinta, G., Hegab, M. M., Pandey, R., Asard, H., & Abuelsoud, W. (2016). High salinity induces different oxidative stress and antioxidant responses in maize seedlings organs. Frontiers in Plant Science, 7,276. https://doi.org/10.3389/fpls.2016.00276
Aebi, H. (1974). Catalae. In H. U. Bergmeyer (Ed.), Methods of enzymatic analysis 2 (pp. 673-684). New York, London: Academic press. https://doi.org/10.1016/B978-0- 12-091302-2.X5001-4
Ahmad, P., Jaleel, C. A., Salem, M. A., Nabi, G., & Sharma, S. (2010). Roles of enzymatic and nonenzymatic antioxidants in plants during abiotic stress. Critical Reviews in Biotechnology, 30(3), 161-175. https://doi.org/10.3109/07388550903524243
de Azevedo Neto, A. D., Prisco, J. T., Enéas-Filho, J., de Abreu, C. E. B., & Gomes-Filho, E. (2006). Effect of salt stress on antioxidative enzymes and lipid peroxidation in leaves and roots of salt-tolerant and salt-sensitive maize genotypes. Environmental and Experimental Botany, 56(1), 87-94. https://doi.org/10.1016/j.envexpbot.2005.01.008
Azooz, M. M., Ismail, A. M., & Elhamd, M. A. (2009). Growth, lipid peroxidation and antioxidant enzyme activities as a selection criterion for the salt tolerance of maize cultivars grown under salinity stress. International Journal of Agriculture & Biology, 11(1), 21-26.
Carrasco-Ríos, L., & Pinto, M. (2014). Effect of salt stress on antioxidant enzymes and lipid peroxidation in leaves in two contrasting corn,’Lluteno’and’Jubilee’. Chilean Journal of Agricultural Research, 74(1), 89-95. http://dx.doi.org/10.4067/S0718-58392014000100014
Esfandiari, E., & Gohari, G. (2017). Response of ROS-scavenging systems to salinity stress in two different wheat (Triticum aestivum L.) cultivars. Notulae Botanicae Horti Agrobotanici Cluj-Napoca, 45(1), 287-291. https://doi.org/10.15835/nbha45110682
Eyer, P., Worek, F., Kiderlen, D., Sinko, G., Stuglin, A., Simeon-Rudolf, V., & Reiner, E. (2003). Molar absorption coefficients for the reduced Ellman reagent: reassessment. Analytical biochemistry, 312(2), 224-227. https://doi.org/10.1016/S0003-2697(02)00506-7
Foyer, C. H., Theodoulou, F. L., & Delrot, S. (2001). The functions of inter-and intracellular glutathione transport systems in plants. Trends in Plant Science, 6(10), 486-492. https://doi.org/10.1016/S1360-1385(01)02086-6
Gill, S. S., Anjum, N. A., Hasanuzzaman, M., Gill, R., Trivedi, D. K., Ahmad, I., Pereira, E., & Tuteja, N. (2013). Glutathione and glutathione reductase: A boon in disguise for plant abiotic stress defense operations. Plant Physiology and Biochemistry, 70, 204-212. https://doi.org/10.1016/j.plaphy.2013.05.032
Gray, J. S., & Montgomery, R. (2003). Purification and characterization of a peroxidase from corn steep water. Journal of Agricultural and Food Chemistry, 51(6), 1592-1601. https://doi.org/10.1021/jf025883n
Hussain, S., Rao, M. J., Anjum, M. A., Ejaz, S., Zakir, I., Ali, M. A., Ahmad, N., & Ahmad, S. (2019). Oxidative stress and antioxidant defense in plants under drought conditions. In M. Hasanuzzaman, K. Hakeem, K. Nahar & H. Alharby (Eds.), Plant abiotic stress tolerance (pp. 207-219). Springer: Cham. https://doi.org/10.1007/978-3-030-06118-0_
Koo, J. S., Choo, Y. S., & Lee, C. B. (2007). Changes in ROS-Scavenging Enzyme Activity in Rice (Oryza sativa L.) Exposed to High Salinity. Journal of Ecology and Environment, 30(4), 307-314. https://doi.org/10.5141/JEFB.2007.30.4.307
Kukavica, B. M., Veljovicć-Jovanovicć, S. D., Menckhoff, L., & Lüthje, S. (2012). Cell wall- bound cationic and anionic class III isoperoxidases of pea root: biochemical characterization and function in root growth. Journal of Experimental Botany, 63(12), 4631-4645. https://doi.org/10.1093/jxb/ers139
Kuvelja, A., Davidović-Plavšić, B., Lukić, D., Gajić, N., Žabić, M., Škondrić, S., & Kukavica, B. (2021). Impact of nicosulfuron on biochemical markers of oxidative stress in maize leaves and roots. Biljni lekar, 49(2), 201-217. https://doi.org/10.5937/biljlek2102201k
Lopez-Huertas, E., Charlton, W. L., Johnson, B., Graham, I. A., & Baker, A. (2000). Stress induces peroxisome biogenesis genes. The EMBO Journal, 19(24), 6770-6777. https://doi.org/10.1093/emboj/19.24.6770
Lowry, O. H., Rosebrough, N. J., Farr, A. L., & Randall, R. J. (1951). Protein measurement with the Folin phenol reagent. Journal of Biological Chemistry, 193, 265-275. PMID: 14907713
Lukić, N., Trifković, T., Kojić, D., & Kukavica, B. (2021). Modulations of the antioxidants defence system in two maize hybrids during flooding stress. Journal of Plant Research, 134(2), 237-248. https://doi.org/10.1007/s10265-021-01264-w
Menezes-Benavente, L., Kernodle, S. P., Margis-Pinheiro, M., & Scandalios, J. G. (2004). Salt- induced antioxidant metabolism defenses in maize (Zea mays L.) seedlings. Redox Report, 9(1), 29-36. https://doi.org/10.1179/135100004225003888
Miyake, C., & Asada, K. (1992). Thylakoid-bound ascorbate peroxidase in spinach chloroplasts and photoreduction of its primary oxidation product monodehydroascorbate radicals in thylakoids. Plant and Cell Physiology, 33(5), 541-553. https://doi.org/10.1093/oxfordjournals.pcp.a078288
Pandey, V. P., Awasthi, M., Singh, S., Tiwari, S., & Dwivedi, U. N. (2017). A comprehensive review on function and application of plant peroxidases. Biochemistry & Analytical Biochemistry, 6(1), 308. DOI: 10.4172/2161-1009.1000308
Polidoros, A. N., & Scandalios, J. G. (1999). Role of hydrogen peroxide and different classes of antioxidants in the regulation of catalase and glutathione S‐transferase gene expression in maize (Zea mays L.). Physiologia Plantarum, 106(1), 112-120. https://doi.org/10.1034/j.1399-3054.1999.106116.x
Sharma, P., Jha, A. B., Dubey, R. S., & Pessarakli, M. (2012). Reactive oxygen species, oxidative damage, and antioxidative defense mechanism in plants under stressful conditions. Journal of Botany, 2012, 217037. https://doi.org/10.1155/2012/217037
Singh, A., Bhushan, B., Gaikwad, K., Yadav, O. P., Kumar, S., & Rai, R. D. (2015). Induced defence responses of contrasting bread wheat genotypes under differential salt stress imposition. Indian Journal of Biochemistry and Biophysics, 52(1), 75-85. PMID: 26040114
Stepien, P., & Klobus, G. (2005). Antioxidant defense in the leaves of C3 and C4 plants under salinity stress. Physiologia Plantarum, 125(1), 31-40. https://doi.org/10.1111/j.1399- 3054.2005.00534.x
Štolfa, I., Pfeiffer, T. Ž., Špoljarić, D., Teklić, T., & Lončarić, Z. (2015). Heavy metal-induced oxidative stress in plants: Response of the antioxidative system. In D. Gupta, J. Palma & F. Corpas (Eds.), Reactive oxygen species and oxidative damage in plants under stress (pp. 127-163). Cham: Springer. https://doi.org/10.1007/978-3-319-20421-5_6
Veljović Jovanović, S., Kukavica, B., Vidović, M., Morina, F., & Menckhoff, L. (2018). Class III peroxidases: Functions, localization and redox regulation of isoenzymes. In D. Gupta, J. Palma & F. Corpas (Eds.), Antioxidants and antioxidant enzymes in higher plants (pp. 269-300). Cham: Springer. https://doi.org/10.1007/978-3-319-75088-0_13
Villalpando-Rodriguez, G. E., & Gibson, S. B. (2021). Reactive oxygen species (ROS) regulates different types of cell death by acting as a rheostat. Oxidative Medicine and Cellular Longevity, 2021, 9912436. https://doi.org/10.1155/2021/9912436
Wang, M., Gong, S., Fu, L., Hu, G., Li, G., Hu, S., & Yang, J. (2022). The involvement of antioxidant enzyme system, nitrogen metabolism and osmoregulatory substances in alleviating salt stress in inbred maize lines and hormone regulation mechanisms. Plants, 11(12), 1547. https://doi.org/10.3390/plants11121547
Wang, Y., Jia, D., Guo, J., Zhang, X., Guo, C., & Yang, Z. (2017). Antioxidant metabolism variation associated with salt tolerance of six maize (Zea mays L.) cultivars. Acta Ecologica Sinica, 37(6), 368-372. https://doi.org/10.1016/j.chnaes.2017.08.007
