BIOCHEMICAL RESPONSES OF PURE AND MIXED FUNGAL CULTURES ISOLATED FROM WASTE WATER TO THE PRESENCE OF SODIUM TRIPOLIPHOSPHATES
DOI:
https://doi.org/10.7251/SKP201101019JKljučne reči:
natrijum tripolifosfat, pH, redoks potencijal, proteolitička aktivnost, T. viride,, G. candidum, mješovita kulturaApstrakt
Natrijum tripolifosfat (Sodium tripolyphosphate STPP) ima široku primjenu u različitim industrijskim procesima, ali uglavnom kao bilder u proizvodima za čišćenje u domaćinstvima. Upotreba STPP povezana je sa ekološkim problemom poznatim kao eutrofikacija. Sa ekološke tačke gledišta, važno je identifikovati mikroorganizme koji imaju sposobnost redukcije STPP iz okoline. U ovom radu je ispitivan hemijski i biohemijski odgovor čistih kultura T. viride i G. candidum i njihove mješovite kulture na prisustvo STPP u koncentraciji 0,5% tokom 8 dana kultivacije u tečnoj hranljivoj podlozi. Sa tim u vezi su ispitivane promjene vrijednosti pH, redoks potencijala, proteolitičke aktivnosti tečnih podloga i ukupne suve mase biomase (dry weight biomass DWB) kultura. Dodatak STPP hranljivoj podlozi uticao je na povećanje početnih vrijednosti pH i smanjenje vrijednosti redoks potencijala u poređenju sa kontrolom. STPP je imao inhibitorno dejstvo na aktivnost proteaza svih kultura: T. viride (3,60%), mješovita kultura (9,77%) i G. candidum (60,33%). Suva biomasa (DWB) T. viride bila je blago inhibirana (1,06%), dok je DWB G. candidum (0,58%) i mješovite kulture (9,53%) blago i srednje stimulisana STPP. Rezultati dobijeni u ovoj studiji ukazuju na potencijalnu ulogu ispitivanih kultura u uklanjanju polifosfata iz postrojenja za prečišćavanje industrijskih i otpadnih voda i njihovu potencijalnu primjenu u biotehnološkim procesima.
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