SADRŽAJ NITRATA I AKTIVNOST NITRAT-REDUKTAZE U POVRĆU UZETOM SA „TRŽNICE“ U BANJOJ LUCI I PROCJENE RIZIKA
DOI:
https://doi.org/10.7251/SKP201101011MKljučne reči:
nitrati, nitrat-reduktaza, povrće, tržnicaApstrakt
Visok sadržaj nitrata i nitrita u povrću može imati toksične i kancerogene efekte na ljudski organizam, te je neophodno praćenje kako bi se primijenile odgovarajuće zaštitne mjere. Cilj istraživanja u ovom radu bio je određivanje sadržaja nitrata i praćenje aktivnosti nitrat-reduktaze u povrću (blitva, mrkva, peršun, kupus) uzetom sa različitih štandova gradske tržnice u Banjoj Luci. Prosječna koncentracija nitrata u uzorcima povrća kretala se od 1 do 650 mgkg-1, pri čemu su najveće vrijednosti dobijene u blitvi i kupusu, a najniže u korijenu peršuna. Aktivnost nitrat-reduktaze bila je povećana u blitvi, dok je najniža utvrđena u korijenu peršuna. Sadržaj nitrata u uzorcima povrća bio je u granicama koje su propisane od strane Svjetske zdravstvene organizacije, tako da ne postoji potencijalna opasnost po zdravlje ljudi.
Reference
Afzali, S. F. i Elahi, R. (2014). Measuring nitrate and nitrite concentrations in vegetables, fruits in Shiraz. Journal of Applied Sciences and Environmental Management, 18(3), 451‒457. doi: 10.4314/jasem.v18i3.12.
Ayaz, A., Topcu, A. i Yurttagul, M. (2007). Survey of nitrate and nitrite levels of fresh vegetables in Turkey. Journal of Food Technology, 5(2), 177‒179. Preuzeto sa: http://docsdrive.com/pdfs/medwelljournals/jftech/2007/177-179.pdf
BfR (Federal Institute for Risk Assessment of Germany) (2009). Nitrate in rocket lettuce, spinach and other lettuces (Opinion No. 032/2009). Preuzeto sa: https://www.bfr.bund.de/cm/349/nitrate_in_rocket_lettuce_spinach_and_other_lettuces.pdf
Chen, B. M., Wang, Z. H., Li, S. X., Wang, G. X., Song, H. X. i Wang, X. N. (2004). Effects of nitrate supply on plant growth, nitrate accumulation, metabolic nitrate concentration and nitrate reductase activity in three leafy vegetables. Plant Science, 167(3), 635‒643. doi: 10.1016/j.plantsci.2004.05.015.
Chung, S. W. C., Tran, J. C. H., Tong, K. S. K., Chen, M. Y. Y., Xiao, Y., Ho, Y. Y. i Chan, C. H. Y. (2011): Nitrate and nitrite levels in commonly consumed vegetables in Hong Kong. Food additives & Contaminants, 4(1), 34‒41. doi: 10.1080/19393210.2011.557784
EFSA, European Food Safety Authority (2008). Nitrate in vegetables. Scientific Opinion of the Panel on Contaminants in the Food Chain (Question No EFSA-Q-2006-071). The EFSA Journal, 689, 1‒79. doi:10.2903/j.efsa.2008.689.
FAO/WHO Joint E pert Committee on Food Additives. Meeting. ( 59th: 2002: Geneva, S itzerland) ( 2003) . Nitrate and nitrite – intake assessment. U: Safety evaluation of certain food additives and contaminants / prepared by the fifty-ninth meeting of the Joint FAO/WHO E pert Committee on Food Additives ( JECFA) .World Health Organization. Preuzeto sa: http:www.inchem.org/documents/jecfa/jecmono/v50je07.htm
Feng, J.-F., Shi, Z., Wu, Y., Wu, H. i Zhao, Y. (2006): Assessment of nitrate exposure in Beijing residents via consumption of vegetables. Chinese Journal of Food Hygiene, 18(6): 514‒516. Preuzeto sa: https://en.cnki.com.cn/Article_en/CJFDTotal-ZSPZ200606006.htm
Gangolli, S. D., Van Den Brandt, P. A., Feron, V. J., Janzowsky, C., Koeman, J. H., Speijers, G. J. A., Spiegelhalder, B., Walker, R. i Wishnok. J. S. (1994). Nitrate, nitrite and N-nitroso compounds. European Journal of Pharmacology: Environmental Toxicology and Pharmacology, 292(1), 1‒38.
Gorenjak, A. H. i Cencič, A. (2013). Nitrate in vegetables and their impact on human health. Acta Alimentaria, 42, 2, 158‒172. doi: 10.1016/0926-6917(94)90022-1
Iammarino, M., Di Taranto, A. i Cristino, M. (2014). Monitoring of nitrites and nitrates levels in leafy vegetables (spinach and lettuce): a contribution to risk assessment. Journal of the Science of Food and Agriculture, 94(4), 773–778. doi: 10.1002/jsfa.6439.
Ivashikina, N. V. i Sokolov, O. A. (1997). Regulation of nitrate distribution in maize seedlings by nitrate, nitrite, ammonium and glutamate. Plant Science, 123(1-2), 29–37. doi: 10.1016/s0168-9452(96)04566
Kastori, R. i Petrović, N. (2003). Nitrati u povrću ‒ fiziološki, ekološki i agrotehnički aspekti. Poljoprivredni fakultet Novi Sad i Naučni institut za ratarstvo i povrtarstvo Novi Sad.
Miloš, S., Hengl, B. i Matijević, L. (2016): Znanstveno mišljenje o riziku od nitrata iz povrća. Osijek, Hrvatska: HAH (Hrvatska agencija za hranu). Preuzeto sa: https://www.hah.hr/wp-content/uploads/2015/10/znanstveno-misljenje-o-riziku-od-nitrata-iz-povrca.pdf
Maksimović, I. i Pajević, S. (2002). Praktikum iz fiziologije biljaka. Univerzitet u Novom Sadu.
Reddy, K. S. i Menary, R. C. (1990). Nitrate reductase and nitrate accumulation in relation to nitrate toxicity in Boronia megastigma. Physiologia Plantarum 78(3), 430‒434. doi:10.1111/j.1399-3054.1990.tb09059.x.
Rezaei, M., Fani, A., Moini, F., Mirzajani, P., Malekirad, A. A. i Rafiei, M. (2014). Determining Nitrate and Nitrite Content in Beverages, Fruits, Vegetables, and Stews Marketed in Arak, Iran. International Scholarly Research Notices, 2014(2), 1‒5. doi:10.1155/2014/439702
Santamaria, P. (2006). Nitrate in vegetables: toxicity, content, intake and EC regulation. Journal of the Science of Food and Agriculture, 86(1), 10‒17. doi:10.1002/jsfa.2351
Shahlaei, A., Ansari, A. N. i Dehkordie, F. S. (2007). Evaluation of nitrate and nitrite content of Iran Southern (Ahwaz) vegetables During Winter and Spring of 2006. Asian Journal of Plant Sciences, 6(8), 1197–1203. doi:10.3923/ajps.2007.1197.1203
Weightman, R. M., Dyer, C., Buxton, J. i Farrington, D. S. (2006). Effects of light level, time of harvest andposition within field on the variability of tissue nitrate concentration in commercial crops of lettuce (Lactuca sativa) and endive (Cichorium endiva). Food Additives & Contaminants, 23(5), 462‒469. doi:10.1080/02652030500522606
