ERYTHROCYTE PROFILES OF RANA DALMATINA AND PELOPHYLAX RIDIBUNDUS (AMPHIBIA: RANIDAE)
DOI:
https://doi.org/10.7251/ASB230402003DKeywords:
frogs, erythrocytes, hemoglobin, hematocrit, hematological indicesAbstract
The research on the erythrocyte profile was conducted on a total of twenty-eight frog individuals, out of which fourteen were Pelophylax ridibundus and also fourteen were Rana dalmatina. The aim of this study was to determine the erythrocyte parameters in the two frog species mentioned, in order to compare the obtained results. The individuals used in the study were captured in the area of Mrkonjić Grad municipality near Lake Balkana. After capture, all individuals were transported to laboratory conditions where they underwent a 15-day adaptation period. Following the adaptation period, blood samples were collected, and erythrocyte parameters were determined, including the number of erythrocytes, hemoglobin concentration, hematocrit and MCV, MCH and MCHC parameters. Based on the obtained results, the study showed that the Pelophylax ridibundus species had significantly higher values of erythrocyte count, hemoglobin concentration and MCHC compared to the values obtained in the Rana dalmatina.
References
Alder, A., & Huber, E. (1923). Unstersucheng uber blutcellen bei Amphibien und Reptilien. Folia Haematologica, 29, 1-22.
Arıkan, H. (1989). Rana ridibunda (Anura, Ranidae) populasyonlarının kan hücrelerinin sayısı bakımından incelenmesi. Turkish Journal of Zoology, 13(2), 54-59.
Arikan, H., Cevik, I. E., Kaya, U., & Mermer, A. (2001). Erythrocytes measurements on Anatolian mountain frogs. Anadolu Ünversitesi Bilim Ve Teknoloji Dergisi, 2, 387-391. Arikan, H., Alpagut-Keskin, N., Çevik, I. E., & Eriomio, U. C. (2010). A study on the blood cells of the fire-bellied toad, Bombina bombina L. (Anura: Bombinatoridae). Animal Biology 60, 61-68. https://doi.org/10.1163/157075610X12610595764174
Arserim, S. K., & Mermer, A. (2008). Hematology of the Uludag Frog, Rana macrocnemis Boulenger, 1885 In Uludag National Park (Bursa, Turkey). Journal of Fisheries of Aquatic Scieces, 25(1), 39-46.
Atatür, M. K., Arıkan, H., & Çevik, I. E. (1999). Erythrocyte sizes of some Anurans from Turkey. Turkey Journal of Zoology, 23, 111-114.
Arvy, L. (1947). Le dimorphisme sexuel sanguin chez Rana temporaria L. et Bufo vulgaris. Laur. Comptes rendus des séances de la Société de biologie et de ses filiales, 141, 457- 459.
Banerjee, V. (1988). Erythrocyte related blood parameters in Bufo melanostictus with reference to sex and body weight. Environment and Ecology, 6, 802-806.
Baraquet, M., Grenat, P. R., Salas, N. E., & Martino, A. L. (2013). Intraspecific variation in erythrocyte sizes among populations of Hypsiboas cordobae (Anura: Hylidae). Acta Herpetologica, 8, 93-97. https://doi.org/10.13128/Acta_Herpetol-12954
Blaxhall, P.C., & Daisley, K.W. (1973). Routine Haematological Methods for Use with Fish Blood. Journal of Fish Biology, 5, 771-781. https://doi.org/10.1111/j.1095- 8649.1973.tb04510.x
Ceccato, E., Cramp, R. L., Seebacher, F., & Franklin, C. E. (2016). Early exposure to ultraviolet-B radiation decreases immune function later in life. Conservation physiology, 4(1):cow037. https://doi.org/10.1093/conphys/cow037
Choubey, B. (1986). Haematological investigation of Himalayan toad Bufo melanostictus
Schneider in relation to sex and size. Biological Bulletin India, 8, 106-114.
Delalić, L., Bajrić, A., Hajdarević, E., Skenderović, I., & Adrović, A. (2022). The blood cell and differential blood count of Bufo bufo from several localities of Tuzla Canton. Acta Scientifica Balcanica, 3(2), 13-22. doi: 10.7251/ASB220302003D
Donmez, F., Tosunoglu, M., & Gul, C. (2009). Hematological values in hermaphrodite, Bufo bufo. North-Western Journal of Zoology, 5(1), 97-103. Article No.: 051111
Garg, A. D., & Hippargi, R. V. (2007). Significance of frogs and toads in environmental conservation. National seminar Eco-environmental impact and organism response, NSEE-IOR'07 (pp. 80-85), 24 February 2007 Nagpur, India.
Gül, Ç., Tosunoğlu, M., Erdoğan, D., & Özdamar, D. (2011). Changes in the blood composition of some anurans. Acta Herpetologica, 6(2), 137-147. https://doi.org/10.13128/Acta_Herpetol-9137
Glomski, C. A., Tamburlin, J., Hard, R., & Chainami, M. (1997). The phylogenetic odyssey of the erythrocyte. IV. The Amphibians. Histology and Histopathology. 12(1), 147-170. PMID: 9046052
Goniakowska, L. (1973). Metabolism, resistance to hypotonic solutions, and ultrastructure of erythrocytes of five amphibian species. Acta Biologica Cracoviensia. Series Zoologia, 16(1), 114-134.
Houlahan, J. E., Findlay, C. S., Schmidt, B. R., Meyers, A. H., & Kuzmin, S. L. (2000). Quantitative evidence for global amphibian population declines. Nature, 404, 752-755. https://doi.org/10.1038/35008052
Hutchinson, V. H., & Szarski, H. (1965). Number of erythrocytes in some amphibians and reptiles, American Society of Amphibians, 30, 373-375.
Kaplan, R. H. (1989). Ovum size plasticity and maternal effects on the early development of the frog, Bombina orientalis, in a field population in Korea. Functional Ecology, 3, 597-604. https://doi.org/10.2307/2389574
Kaplan, H. M. (1951). A study of frog blood in red leg disease. Trans. III. Ilinois Academy of Science Transactions, 44, 209-215.
Kaplan, H. M. (1952). Variations in white blood cells between normal and red leg frogs. Illinois Academy of Science Transactions, 45, 170 176.
Kaya, U., Kuzmin, S., Sparreboom, M., Ugurtas, I. H., Tarkhnishvili, D., Anderson, S., Andreone, F., Corti, C., Nyström, P., Schmidt, B., Anthony, B., Ogrodowczyk, A., Ogielska, M., Bosch, J., & Tejedo, M. (2009). Rana dalmatina. The IUCN Red List of Threatened Species 2009:e.T58584A11790570. http://dx.doi.org/10.2305/IUCN.UK.2009.RLTS.T58584A1179 0570.en
Kekić, H., & Ivanc, A. (1982). A new direct method for counting fish blood cells. Ichtyologia, 14(1), 55-61.
Kuzmin, S., Tarkhnishvili, D., Ishchenko, V., Dujsebayeva, T., Tuniyev, B.,Papenfuss, T., Beebee, T., Ugurtas, I.H., Sparreboom, M., Rastegar-Pouyani, N., Mousa Disi, A. M., Anderson, S., Denoel, M., & Andreone, F. (2009). Pelophylax ridibundus. The IUCN Red List of Threatened Species. https://dx.doi.org/10.2305/IUCN.UK.2009.RLTS.T58705A11825745.en
Klieneberger, C. (1927). Die Blutmorphologie d. Laboratoriumstiere. Liepzig: Barth.
Nicolas, A. E. (2003). Reptiles and Amphibians of Europe. Princeton Field Guides, 24. Princeton University Press.
Özgül, C. N., Kurtul, D., & Gül, C. (2020). Hematologic and Genotoxicological Research on Pelophylax ridibundus and Bufotes variabilis Living Around the Çan (Çanakkale, Turkey). Turkish Journal of Bioscience and Collections, 4(2), 105-111. DOI:10.26650/tjbc.20200011
Prokić, M. D. (2016). Parametri antioksidacionog sistema u tkivima žaba Pelophylax esculentus kompleksa kao biomarkeri izlaganja metalima. Doktorska disertacija. Beograd: Biološki fakultet, Univerzitet u Beogradu.
Rouf, M. A. (1969). Hematology of the lepoard frog, Rana pipiens. Copeia, 4, 682-687.
Ruiz, G. (1983). Respiratory and hematological adaptations to high altitude in Telmatobius frogs from the Chilean Andes. Comparative Biochemistry and Physiology Part A: Physiology, 76(1), 109-113. https://doi.org/10.1016/0300-9629(83)90300-6
Samantaray, K. (1985). Studies on hematology of Indian skipper frog, Rana cyanophlyctis
Schneider. Comparative Physiology and Ecology, 10, 71-74.
Schermer, S. (1954). Die Bluttmorphologie der Laboratoriumstiere. Leipzig: Barth.
Sinha, R. C. (1983). Haematological studies on the prewintering and wintering frog, Rana esculenta. Comparative Biochemistry and Physiology, 74A, 311-314. https://doi.org/10.1016/0300-9629(83)90606-0
Stephan. J. (1954). Morphologie générale du système circulatoire. In: Grassé, P. P. (Ed.), Traité de zoologie, tome XII, 942–946. Paris: Masson.
Thammachoti, P., Khonsue, W., Kitana, J., & Varanusupakul, P. (2012). Morphometric and gravimetric parameters of the rise frog Fejervarya limnocharis living in areas with different agricultural activity. Journal of Environmental Protection, 3(10), 1403-1408. DOI: 10.4236/jep.2012.310159
Wojtaszek, J., Baranowska, M., Glubiak, M., & Dżugaj, A. (1997). Circulating blood parameters of the water frog, Rana esculenta at pre-wintering stage. Zoologica Poloniae, 42(1–4), 117-126.
Whittaker, K., Koo, M. S., Wake, D. B., & Vredenburg, V. T. (2013). Global declines of amphibians. In: Levin, S. A. (Ed.), Encyclopedia of Biodiversity. 2nd (pp. 691-699). Cambridge: MA, USA: Elsevier Incorporation (Academic Press).
Zhukova, T. I., & Kubantsev, B. S. (1979). Changes in the blood composition of salientians during hibernation. Soviet Journal of Ecology, 9, 379-382.
Zhelev, Z., Arnaudovab, D., Popgeorgievc, G., & Tsonev, S. (2020). In situ assessment of health status and heavy metal bioaccumulation of adult Pelophylax ridibundus (Anura: Ranidae) individuals inhabiting polluted area in southern Bulgaria. Ecological Indicators, 115, 106413. https://doi.org/10.1016/j.ecolind.2020.106413
Šorić, V. (1997). Morfologija i sistematika hordata. Kragujevac: Prirodno-matematički fakultet.
